Improvement of Immune Dysfunction in Dogs With Multiple Organ Dysfunction by High-volume Hemofiltration

Authors

  • Xiaolu Sui Department of Nephrology, The First Hospital Affiliated of Xinjiang Medical University, Urumqi, China Author
  • Yunpeng Xu Department of Nephrology, The First Hospital Affiliated of Xinjiang Medical University, Urumqi, China Author
  • Aisha Zhang Department of Nephrology, The First Hospital Affiliated of Xinjiang Medical University, Urumqi, China Author
  • Xuehua Wang Xinyuan County People's Hospital, Yili, China Author
  • Lei Ye Department of Nephrology, The First Hospital Affiliated of Xinjiang Medical University, Urumqi, China Author
  • Wenjun Yang Department of Nephrology, The First Hospital Affiliated of Xinjiang Medical University, Urumqi, China Author
  • Bing Ma Department of Nephrology, The First Hospital Affiliated of Xinjiang Medical University, Urumqi, China Author
  • Jingjing Zhang Department of Nephrology, The First Hospital Affiliated of Xinjiang Medical University, Urumqi, China Author
  • Jihong Chen Department of Nephrology, The First Hospital Affiliated of Xinjiang Medical University, Urumqi, China Author

Abstract

Introduction. Observing the effects of high-volume hemofiltration (HVHF) treatment on the monocytes apoptosis, antigen presentation, and secretion function, this study investigated the mechanism of HVHF effect on immunity homeostasis in multiple organ dysfunction syndrome (MODS) in an animal model.

Materials and Methods. Lipopolysaccharides were administered in 12 Beagle dogs in order to induce MODS. Six dogs were randomly assigned to receive HVHF treatment for 12 hours (HVHF group) and the rest did not receive any treatment (the MODS group). The expression of DLA-DR, apoptosis, and cytokine levels were measured at 7 time points: normal condition (T1), after operation (T2), and zero, 3, 6, 9, and 12 hours after endotoxin injection (T3 to T7, respectively).

Results. Apoptosis of CD14+ mononuclear cell increased in early and late stages gradually in the MODS group and began to decline gradually after the HVHF treatment. There was a significant difference between the two groups at time points T2 to T7 (P < .01). After HVHF, the impaired expression of dog leukocyte antigen-DR showed an improvement trend in the HVHF group, which was significant better at T5 and T7 than that in the MODS group (P < .05). Interleukin-4 secretion increased significantly with HVHF and was significantly higher at time points T4 to T7 as compared with the MODS group (P < .01). 

Conclusions. High-volume hemofiltration can alleviate the mononuclear cell apoptosis, improve antigen-presenting function and secretion function, inhibit the release of inflammatory factors, and maintain immune homeostasis, and consequently alleviate symptoms of MODS effectively.

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Author Biographies

  • Xiaolu Sui, Department of Nephrology, The First Hospital Affiliated of Xinjiang Medical University, Urumqi, China

    Department of Nephrology, the first hospital affiliated of Xinjiang Medical University, Urumqi, China

  • Aisha Zhang, Department of Nephrology, The First Hospital Affiliated of Xinjiang Medical University, Urumqi, China

     In this study, Aisha Zhang made the same contribution as the first author.

  • Xuehua Wang, Xinyuan County People's Hospital, Yili, China

    Xinyuan County People's Hospital, Yili, China.

  • Lei Ye, Department of Nephrology, The First Hospital Affiliated of Xinjiang Medical University, Urumqi, China

    Department of Nephrology, the first hospital affiliated of Xinjiang Medical University, Urumqi, China.

  • Wenjun Yang, Department of Nephrology, The First Hospital Affiliated of Xinjiang Medical University, Urumqi, China

    Department of Nephrology, the first hospital affiliated of Xinjiang Medical University, Urumqi, China.

  • Bing Ma, Department of Nephrology, The First Hospital Affiliated of Xinjiang Medical University, Urumqi, China

    Department of Nephrology, the first hospital affiliated of Xinjiang Medical University, Urumqi, China.

  • Jingjing Zhang, Department of Nephrology, The First Hospital Affiliated of Xinjiang Medical University, Urumqi, China

    Department of Nephrology, the first hospital affiliated of Xinjiang Medical University, Urumqi, China.

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Published

2015-09-02

Issue

Section

ORIGINAL | Kidney Diseases

How to Cite

Improvement of Immune Dysfunction in Dogs With Multiple Organ Dysfunction by High-volume Hemofiltration. (2015). Iranian Journal of Kidney Diseases, 9(6), 433-439. https://www.ijkd.org/index.php/ijkd/article/view/1958