Role of Donors and Recipients’ Glutathione S-Transferase Gene Polymorphisms in Association of Oxidative Stress With Delayed Graft Function in Kidney Allograft Recipients

Authors

  • Jalal Azmandian Physiology Research Centre, Institute of Neuropharmacology; Department of Nephrology, Urology and Renal Transplantation, Afzalipoor Hospital, Kerman University of Medical Sciences, Kerman, Iran
  • Ali Mandegary Gastroenterology and Hepatology Research Centre, Institute of Basic and Clinical Physiology Sciences; Department of Pharmacology and Toxicology, School of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran
  • Mahboobeh Pootari Pharmaceutics Research Centre, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
  • Mohamad-Hadi Nematolahi Research Center for Hydatid Disease in Iran, University of Medical Sciences, Kerman, Iran
  • Mohammad-Reza Ebadzadeh Physiology Research Centre, Institute of Neuropharmacology; Department of Nephrology, Urology and Renal Transplantation, Afzalipoor Hospital, Kerman University of Medical Sciences, Kerman, Iran
  • Simin-Dokht Habibzadeh Department of Nephrology, Urology and Renal Transplantation, Afzalipoor Hospital, Kerman University of Medical Sciences, Kerman, Iran
  • Mohammad-Hassan Dehghani-Firouzabadi Department of Nephrology, Urology and Renal Transplantation, Afzalipoor Hospital, Kerman University of Medical Sciences, Kerman, Iran
  • Abbas Etminan Department of Nephrology, Urology and Renal Transplantation, Afzalipoor Hospital, Kerman University of Medical Sciences, Kerman, Iran
  • Faramarz Fazeli Department of Urology, Zahedan University of Medical Sciences, Zahedan, Iran
  • Maryamalsadat Mousavi Department of Urology, Zahedan University of Medical Sciences, Zahedan; Chronic Kidney Disease Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Abstract

Introduction. Oxidative stress contributes to delayed graft function (DGF). Glutathione S-transferases (GSTs) are polymorphic genes which produce enzymes with protective effect against oxidative stress. This study aimed to investigate the association between donors' and recipients' GSTM1 and GSTT1 polymorphisms and DGF, creatinine clearance, and oxidative stress parameters in kidney allograft recipients.

Materials and Methods. One hundred and eighty-two donor-recipient pairs were studied. Lipid peroxidation and total antioxidant capacity were measured in the recipients' plasma as the parameters of oxidative stress. Delayed graft function was determined based on at least 10% increase, no change, or less than 10% decrease in the serum creatinine level in 3 consecutive days during the 1st week after transplantation.

Results. Lipid peroxidation was significantly greater in the recipients with DGF (P < .001). The frequency of GSTM1 null was significantly higher in the patients with DGF (odds ratio [OR], 0.38; 95% confidence interval [CI], 0.17 to 0.86; P = .02). There was also a significant association between the donors' GSTM1 polymorphism and DGF (OR, 0.31; 95% CI, 0.14 to 0.68; P = .003). A significant association was detected between combination of recipients and donors' GSTM1 polymorphism and DGF (OR, 0.20; 95% CI, 0.07 to 0.64, P = .006). The recipients' GSTM1 polymorphism, alone and in combination with donors' GSTM1 and GSTT1, significantly affected the creatinine clearance on discharge day.

Conclusions. These results suggest that the donors and recipients' GSTM1 polymorphism may be a major risk factor for oxidative stress and poor kidney allograft transplantation outcomes.

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Published

2017-05-31

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Section

ORIGINAL | Transplantation