Upregulation of Cabin1 During Injury to Renal Tubular Epithelial Cells in Rats

Authors

  • Yueqiang Wen Department of Nephrology, The Second Affiliated Hospital, Guangzhou Medical University, Guangzhou, China Author
  • Huiyuan Li Department of Nephrology, The Second Affiliated Hospital, Guangzhou Medical University, Guangzhou, China Author
  • Peilan Zhou Department of Nephrology, The Second Affiliated Hospital, Guangzhou Medical University, Guangzhou, China Author
  • Lingling Liu Department of General Medicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China Author
  • Zebin Wang Department of Nephrology, The Second Affiliated Hospital, Guangzhou Medical University, Guangzhou, China Author
  • Jianbo Liang Department of Nephrology, The Second Affiliated Hospital, Guangzhou Medical University, Guangzhou, China Author

Abstract

Introduction. Calcineurin-binding protein 1 (Cabin1) interacts with calcineurin and p53, but its function in renal tubular epithelial cell (RTEC) is unclear. We established 5/6 nephrectomized rats and angiotensin II-induced injury to the RTECs in vitro, to observe the expression of Cabin1 during RTEC injury.

Materials and Methods. Sprague-Dawley rats were sacrificed at 4 and 8 weeks after 5/6 nephrectomy. Renal pathology and mitochondrial damage were detected by light and electrical microscope. The distribution of E-cadherin and α-smad were detected by indirect immunofluorescence staining. Cabin1 protein expression was detected by Western blot.

Results. Obvious tubulointerstitial fibrosis was found in the nephrectomized rats at 8 weeks after 5/6 nephrectomy, accompanied by the increasing levels of creatinine, as well as the disruption of E-cadherin and overexpression of α-smad in RTECs. Moreover, the mitochondria became swollen and mitochondrial cristae were disrupted and poorly defined in the RTECs. Compared to the sham-operated rats, Cabin1 protein expression was significantly increased at 8 weeks after 5/6 nephrectomy, while angiotensin II-induced Cabin1 protein expression significantly increased 48 hours after stimulation in normal rat kidney epithelial cells.

Conclusions. Injury to the RTEC and Cabin1 protein overexpression occurred in a time-dependent manner both in vitro and in vivo. Cabin1 may become a potential molecular target in RTEC injury.

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Published

2017-11-29

Issue

Section

ORIGINAL | Kidney Diseases

How to Cite

Upregulation of Cabin1 During Injury to Renal Tubular Epithelial Cells in Rats. (2017). Iranian Journal of Kidney Diseases, 11(6), 430-437. https://www.ijkd.org/index.php/ijkd/article/view/3183

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