A Combination of Vitamin C and Losartan for Cisplatin-induced Nephrotoxicity in Rats

Authors

  • Farzaneh Ashrafi Water and Electrolytes Research Center and Department of Internal Medicine-Haematology Section, Isfahan University of Medical Sciences, Isfahan, Iran
  • Mehdi Nematbakhsh Water and Electrolytes Research Center, Kidney Diseases Research Center, and Department of Internal Medicine-Nephrology Section, Isfahan University of Medical Sciences, Isfahan, Iran
  • Tahereh Safari Water and Electrolytes Research Center and Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran
  • Ardeshir Talebi Water and Electrolytes Research Center and Department of Clinical Pathology, Isfahan University of Medical Sciences, Isfahan, Iran
  • Hamid Nasri Water and Electrolytes Research Center and Department of Internal Medicine-Haematology Section, Isfahan University of Medical Sciences, Isfahan, Iran
  • Mehdi Khazaei Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran
  • Mohammad-Mehdi Baradaran-Mahdavi Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran
  • Amir Jafapisheh Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran
  • Behrooz Olia Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran
  • Omid Pirhaji Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran
  • Sayyed-Javad Hashemi-Nia Department of Immunology, Isfahan University of Medical Sciences, Isfahan, Iran
  • Fatemeh Eshraghi Department of Immunology, Isfahan University of Medical Sciences, Isfahan, Iran
  • Zahra Pezeshki Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  • Mojgan Mortazavi Kidney Diseases Research Center and Department of Internal Medicine-Nephrology Section, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Introduction. The nephroprotective effect of co-administration of vitamin C and losartan as prophylaxis against cisplatin-induced nephrotoxicity (CIN) was evaluated.

Materials and Methods. Co-administration of vitamin C and losartan was compared with losartan (10 mg/kg), vitamin C (250 mg/kg), and placebo in 4 groups of rats with CIN. The prophylactic agents were injected daily for a period of 4 days, and on day 3, a single dose (6 mg/kg) of cisplatin was administrated. The animals were sacrificed 7 days later for pathological examination of the kidneys.

Results. Cisplatin prevented the animals' weight gain. The serum levels of creatinine and blood urea nitrogen increased within the groups with CIN, but no significant difference was observed between the groups. The prophylaxis has no effect on serum osmolality, total protein, or nitrite concentrations. The kidney tissue damage was scored, and losartan provided a lower damage score than vitamin C and a combination of vitamin C and losartan.

Conclusions. We concluded that co-administration of vitamin C and losartan was not more effective than the administration of vitamin C or losartan alone.

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Published

2012-09-11

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Section

ORIGINAL | Kidney Diseases